LymphedemaV1, Main, Exploration, bibRecord, 007817

Transcapillary fluid balance consequences of missing initial lymphatics studied in a mouse model of primary lymphoedema

Identifieur interne : 007817 ( Main/Exploration ); précédent : 007816; suivant : 007818

Transcapillary fluid balance consequences of missing initial lymphatics studied in a mouse model of primary lymphoedema

Auteurs : Tine V. Karlsen ; Marika J. Karkkainen ; Kari Alitalo [Finlande] ; Helge Wiig

Source :

The Journal of Physiology [ 0022-3751 ] ; 2006.

RBID : PMC:1817763

Descripteurs français

KwdFr :
- Animaux, Femelle, Homéostasie (physiologie), Inflammation, Interleukine-4 (physiologie), Liquide extracellulaire (physiologie), Lymphangiogenèse (génétique), Lymphangiogenèse (physiologie), Lymphoedème (anatomopathologie), Lymphoedème (génétique), Lymphoedème (physiopathologie), Modèles animaux de maladie humaine, Mâle, Phénotype, Souches mutantes de souris, Souris, Souris transgéniques, Système lymphatique (anatomopathologie), Système lymphatique (physiopathologie), Vaisseaux lymphatiques (anatomopathologie), Vaisseaux lymphatiques (physiopathologie), Équilibre hydroélectrolytique (physiologie).
MESH :
- anatomopathologie : Lymphoedème, Système lymphatique, Vaisseaux lymphatiques.
- génétique : Lymphangiogenèse, Lymphoedème.
- physiologie : Homéostasie, Interleukine-4, Liquide extracellulaire, Lymphangiogenèse, Équilibre hydroélectrolytique.
- physiopathologie : Lymphoedème, Système lymphatique, Vaisseaux lymphatiques.
- Animaux, Femelle, Inflammation, Modèles animaux de maladie humaine, Mâle, Phénotype, Souches mutantes de souris, Souris, Souris transgéniques.

English descriptors

KwdEn :
- Animals, Disease Models, Animal, Extracellular Fluid (physiology), Female, Homeostasis (physiology), Inflammation, Interleukin-4 (physiology), Lymphangiogenesis (genetics), Lymphangiogenesis (physiology), Lymphatic System (pathology), Lymphatic System (physiopathology), Lymphatic Vessels (pathology), Lymphatic Vessels (physiopathology), Lymphedema (genetics), Lymphedema (pathology), Lymphedema (physiopathology), Male, Mice, Mice, Mutant Strains, Mice, Transgenic, Phenotype, Water-Electrolyte Balance (physiology).
MESH :
- chemical , physiology : Interleukin-4.
- genetics : Lymphangiogenesis, Lymphedema.
- pathology : Lymphatic System, Lymphatic Vessels, Lymphedema.
- physiology : Extracellular Fluid, Homeostasis, Lymphangiogenesis, Water-Electrolyte Balance.
- physiopathology : Lymphatic System, Lymphatic Vessels, Lymphedema.
- Animals, Disease Models, Animal, Female, Inflammation, Male, Mice, Mice, Mutant Strains, Mice, Transgenic, Phenotype.

Abstract

To investigate the phenotypic consequences of a deranged lymphangiogenesis in relation to tissue fluid accumulation and the possible role of inflammation in the pathogenesis of lymphoedema, we measured determinants of transcapillary fluid filtration and inflammatory mediators in the interstitial fluid in genetically engineered Chy mice, a model for primary congenital lymphoedema (Milroy's disease). Although initial lymphatics were not present in dermis in any of the areas studied (fore paw, hind paw, thigh and back skin) interstitial fluid pressure (P_if), measured with micropipettes, and tissue fluid volumes were significantly increased only in the areas with visible swelling – the fore and hind paw, whereas interstitial colloid osmotic pressure (COP_if) was increased in all the skin areas examined. A volume load of 15% of body weight resulted in a more pronounced increase in P_if as well as a four-fold increase in interstitial fluid volume in Chy relative to wild-type (wt) mice, showing the quantitative importance of lymphatics for fluid homeostasis during acute perturbations. A similar level of proinflammatory markers in interstitial fluid in early established lymphoedema (3–4 months) in Chy and wt suggests that inflammation does not have a major pathogenetic role for the development of lymphoedema, whereas a reduced level of the immunomodulatory cytokine interleukin (IL)-4 may result in a reduced immunological defence ability and thus lead to the increase in inflammatory cytokines IL-2 and IL-6 observed at a later stage (11–13 months). Our data suggest that primary lymphoedema results in a high interstitial fluid protein concentration that does not induce an interstitial inflammatory reaction per se, and furthermore shows the paramount importance of the initial lymphatics in tissue fluid homeostasis, especially during perturbations of transcapillary fluid balance.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1817763

DOI: 10.1113/jphysiol.2006.108308
PubMed: 16675495
PubMed Central: 1817763

Affiliations:

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<term>Female</term>
<term>Homeostasis (physiology)</term>
<term>Inflammation</term>
<term>Interleukin-4 (physiology)</term>
<term>Lymphangiogenesis (genetics)</term>
<term>Lymphangiogenesis (physiology)</term>
<term>Lymphatic System (pathology)</term>
<term>Lymphatic System (physiopathology)</term>
<term>Lymphatic Vessels (pathology)</term>
<term>Lymphatic Vessels (physiopathology)</term>
<term>Lymphedema (genetics)</term>
<term>Lymphedema (pathology)</term>
<term>Lymphedema (physiopathology)</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Mutant Strains</term>
<term>Mice, Transgenic</term>
<term>Phenotype</term>
<term>Water-Electrolyte Balance (physiology)</term>
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<term>Femelle</term>
<term>Homéostasie (physiologie)</term>
<term>Inflammation</term>
<term>Interleukine-4 (physiologie)</term>
<term>Liquide extracellulaire (physiologie)</term>
<term>Lymphangiogenèse (génétique)</term>
<term>Lymphangiogenèse (physiologie)</term>
<term>Lymphoedème (anatomopathologie)</term>
<term>Lymphoedème (génétique)</term>
<term>Lymphoedème (physiopathologie)</term>
<term>Modèles animaux de maladie humaine</term>
<term>Mâle</term>
<term>Phénotype</term>
<term>Souches mutantes de souris</term>
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<term>Souris transgéniques</term>
<term>Système lymphatique (anatomopathologie)</term>
<term>Système lymphatique (physiopathologie)</term>
<term>Vaisseaux lymphatiques (anatomopathologie)</term>
<term>Vaisseaux lymphatiques (physiopathologie)</term>
<term>Équilibre hydroélectrolytique (physiologie)</term>
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<term>Vaisseaux lymphatiques</term>
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<term>Lymphoedème</term>
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<term>Interleukine-4</term>
<term>Liquide extracellulaire</term>
<term>Lymphangiogenèse</term>
<term>Équilibre hydroélectrolytique</term>
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<term>Homeostasis</term>
<term>Lymphangiogenesis</term>
<term>Water-Electrolyte Balance</term>
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<term>Système lymphatique</term>
<term>Vaisseaux lymphatiques</term>
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<front><div type="abstract" xml:lang="en"><p>To investigate the phenotypic consequences of a deranged lymphangiogenesis in relation to tissue fluid accumulation and the possible role of inflammation in the pathogenesis of lymphoedema, we measured determinants of transcapillary fluid filtration and inflammatory mediators in the interstitial fluid in genetically engineered Chy mice, a model for primary congenital lymphoedema (Milroy's disease). Although initial lymphatics were not present in dermis in any of the areas studied (fore paw, hind paw, thigh and back skin) interstitial fluid pressure (<italic>P</italic>
<sub>if</sub>
), measured with micropipettes, and tissue fluid volumes were significantly increased only in the areas with visible swelling – the fore and hind paw, whereas interstitial colloid osmotic pressure (COP<sub>if</sub>
) was increased in all the skin areas examined. A volume load of 15% of body weight resulted in a more pronounced increase in <italic>P</italic>
<sub>if</sub>
 as well as a four-fold increase in interstitial fluid volume in Chy relative to wild-type (wt) mice, showing the quantitative importance of lymphatics for fluid homeostasis during acute perturbations. A similar level of proinflammatory markers in interstitial fluid in early established lymphoedema (3–4 months) in Chy and wt suggests that inflammation does not have a major pathogenetic role for the development of lymphoedema, whereas a reduced level of the immunomodulatory cytokine interleukin (IL)-4 may result in a reduced immunological defence ability and thus lead to the increase in inflammatory cytokines IL-2 and IL-6 observed at a later stage (11–13 months). Our data suggest that primary lymphoedema results in a high interstitial fluid protein concentration that does not induce an interstitial inflammatory reaction <italic>per se</italic>
, and furthermore shows the paramount importance of the initial lymphatics in tissue fluid homeostasis, especially during perturbations of transcapillary fluid balance.</p>
</div>
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<name sortKey="Karlsen, Tine V" sort="Karlsen, Tine V" uniqKey="Karlsen T" first="Tine V" last="Karlsen">Tine V. Karlsen</name>
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<country name="Finlande"><region name="Uusimaa"><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
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Serveur d'exploration sur le lymphœdème

Transcapillary fluid balance consequences of missing initial lymphatics studied in a mouse model of primary lymphoedema

Transcapillary fluid balance consequences of missing initial lymphatics studied in a mouse model of primary lymphoedema

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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